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. 2018 May 29;9:1112. doi: 10.3389/fmicb.2018.01112

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Copyright © 2018 Kim, Yoon, Ham, Seok and Park.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of PykA activity and pyruvate on resistance of V. vulnificus to H₂O₂ stress. (A) Growth curves of wild-type CMCP6 (squares), pykA mutant (triangles) and complemented (pykA/pRK-PKA, circles) strains in LB supplemented with 2.5% NaCl (LBS medium) in the presence of 0.8 mM H₂O₂. (B) The relative expression levels of catalase in wild-type CMCP6 (lanes 1 and 4), pykA mutant (lanes 2 and 5) and the complemented strains (lanes 3 and 6) in LBS medium in the presence and absence of 0.8 mM H₂O₂. Same amounts of proteins in cell lysates were loaded in each lane. (C) Stationary-phase cells of the indicated V. vulnificus strains were serially diluted fourfold from 0.25 × 10⁸ cells/ml and 2-μl aliquots were spotted onto LBS medium containing different combinations of H₂O₂ and sodium pyruvate as indicated. Representative data from at least three independent experiments are presented.