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The RNA of MERS-CoV was used to evaluate the RT-LAMP-VF assay. (A) Ten-fold serial dilutions of MERS-CoV RNA were detected with the RT-LAMP-VF assay. (B) The RT-LAMP-VF assay was performed on mixtures in which the dilutions of MERS-CoV RNA were spiked into the total RNA of throat swabs collected from healthy people.
