Table 4.

Reaction times and concentrations for inner primer optimization for RT-LAMP.

Time/min	FIP/BIP concentration (μM)	Recombinant plasmid dilution (2 × copies/μl)
		107	106	105	104	103	102	101	100	N
30	0.2	+	+	+	+	+	-	-	-	-
	0.4	+	+	+	+	+	+	-	-	-
40	0.2	+	+	+	+	+	+	-	-	-
	0.4	+	+	+	+	+	+	-	-	-
50	0.2	+	+	+	+	+	+	+	-	-
	0.4	+	+	+	+	+	+	+	-	-

The serially diluted 10-fold recombinant plasmids were detected at three different times at 65°C. Moreover, the amplification was performed at different concentration for each of inner primers FIP and BIP (0.2 or 0.4 μM). Three replications were performed for each trial.