Table 5.

Reaction temperature optimization for RT-LAMP.

Temperature/°C	Synthesized RNA transcript dilution (2 × copies/μl)
	107	106	105	104	103	102	101	100	N
65	+	+	+	+	-	-	-	-	-
63	+	+	+	+	+	-	-	-	-
61	+	+	+	+	+	+	-	-	-
59	+	+	+	+	+	+a	-	-	-

^aThe result was weakly positive. The serially diluted 10-fold synthesized RNA transcripts were detected at four different temperatures for 30 min. Three replications were performed for each trial.