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. 2018 May 29;9:672. doi: 10.3389/fpls.2018.00672

FIGURE 2.

FIGURE 2

TUNEL test illustrating programmed DNA fragmentation (a–d) and phenotype symptoms (e–h) on potato leaves inoculated with P. infestans at 48 hpi. Representative images of TUNEL-positive nuclei (a) and HR-like lesions (e) observed during the avr P. infestans-potato interaction; TUNEL-positive reaction in leaves of potato pretreated with epicatechin and next inoculated with avr P. infestans (b); TUNEL-positive reaction in leaves of potato pretreated with ebselen and next inoculated with avr P. infestans (c); TUNEL-negative reaction (d) and disease spots (h) observed during the vr P. infestans-potato interaction. Bars indicate 15 μm (a–d) and 500 μm (e–h). The percentage of leaf cells exhibiting the TUNEL-positive reaction at 48 hpi (i), 100 cells from at least 5 randomly selected slides were examined at each time point per treatment. Values represent the mean ± SD of four independent experiments (n = 20). Asterisks indicate values that differ significantly from P. infestans inoculated potato leaves at P < 0.05 (∗).