Skip to main content
. 2018 May 29;9:672. doi: 10.3389/fpls.2018.00672

FIGURE 3.

FIGURE 3

RNA and mRNA nitration in healthy and challenge inoculated leaves of potato. 8-Nitroguanine ELISA standard curve (A) in the concentration range of 0 ng/mL to 1000 ng/mL; (B) quantification of nitrated RNA measured as 8-NO2-G content in resistant and susceptible healthy potato leaves enriched with ONOO-; 8-NO2-G content was estimated 5 h after leaf pretreatment with 50 μM SIN-1. Quantification of nitrated RNA measured as 8-NO2-G content in leaves of resistant (C) and susceptible (D) potato inoculated with P. infestans. Quantification of nitrated mRNA measured as 8-NO2-G content in leaves of resistant (E) and susceptible (F) potato inoculated with P. infestans. Analyses were performed at 1, 3, 6, 24, 48, 72, and 96 h after challenge inoculation. Values represent the mean ± SD of at least three independent experiments (n = 9). Asterisks indicate values that differ significantly from mock inoculated (control) potato leaves at ∗P < 0.05.