The expression of transforming growth factor-beta 1 (TGF-β1), alpha-smooth muscle actin (α-SMA) and Tenascin-C (TNC) genes was analyzed by real-time polymerase chain reaction (PCR) after a) three days and b) seven days of cultivation. Data represent mean and standard deviation (n = 6, *p < 0.05, †p < 0.01; one-way analysis of variance). AFib, arthrofibrotic tissue; Fib, fibroblasts isolated from healthy tissue.