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. 2018 May 5;7(4):289–297. doi: 10.1302/2046-3758.74.BJR-2017-0302.R1

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© 2018 Author(s) et al.

This is an open-access article distributed under the terms of the Creative Commons Attributions licence (CC-BY-NC), which permits unrestricted use, distribution, and reproduction in any medium, but not for commercial gain, provided the original author and source are credited.

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graphic file with name bonejointres-07-289-g002.jpg — a) Alizarin staining to show osteogenic differentiation of young, adult, and ovariectomized (OVX) mesenchymal stem cells (MSCs) at day seven, 14, and 21. b) Graph showing the mean Alizarin Red production when MSCs from young, adult control, and OVX rats (n = 3) differentiated to osteoblasts at days seven, 14, and 21. *Significant difference of p < 0.05 using a two-tailed Student’s t-test.