Skip to main content
. 2018 May 29;9:1186. doi: 10.3389/fimmu.2018.01186

Figure 5.

Figure 5

Nitric oxide (NO) released by tumor-associated macrophages reduces syntaxin 4 (synt4) expression in tumor cells. Synt4 mRNA expression in (A) U373 and (B) GL261 cells treated with DETA-NO (20 µM, 1 h) and 8Br-cyclic GMP (cGMP) (3 mM, 1 h), assessed by real-time PCR (n = 3). Data are expressed as the fold change over untreated (UT) controls (dashed line). Synt4 protein expression in (C) U373 and (D) GL261 cells treated with DETA-NO (20 µM, 1 h) and 8Br-cGMP (3 mM, 1 h), or (E) GL261 cells cultured for 24 h in the presence of wild-type (wt) M2 and iNOS−/− M2 macrophages, assessed by western blotting. The images are representative of three independent experiments. Panel on the right shows the densitometry analysis of synt4 expression. Data are expressed as the fold change over UT controls (dashed line). *p < 0.05, **p < 0.001 vs UT. (F) Synt4 protein expression of Lewis lung carcinoma flank allografts at 20 days after injection in wt and iNOS−/− mice, assessed by western blotting. The images are representative of 3–5 independent experiments. Panel on the right shows the densitometry analysis of synt4 expression. Data are showed using stain-free total proteins staining as loading control. *p < 0.05 vs wt.