Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 4;217(6):1957–1972. doi: 10.1083/jcb.201611087

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Bobkov et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure 6. — Centromere association of CAL1 is less stable than CENP-C, and neither CAL1 nor CENP-C is sensitive to transcriptional inhibition. (A) Western blot analysis showing the displacement of CAL1 from chromatin after high-salt extraction. Arrows mark protein of interest. Endogenous dCENP-A serves as a marker for chromatin. (B) Maximum-intensity projection of cells fixed after 30 min of 0.5 M salt extraction and immunostained for CAL1 and dCENP-C. Extracted cells with clustering of centromeres still intact (upper panel) and disrupted (lower panel) are shown. Bars, 3 µm. (C) Quantifications of centromeric localization of total dCENP-A, dCENP-C, and CAL1 in inhibitor-treated cells. Fixation type and group of analyzed cells is indicated above. n = 3 replicates; n = 25–50 cells. Data are mean + SD.