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. 2018 Jun 4;217(6):2033–2046. doi: 10.1083/jcb.201703205

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© 2018 Iuliano et al.

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Figure 7. — Myo1b expression regulates the trafficking of Kif5C⁵⁶⁰ in neurites. The dynamics of Kif5C⁵⁶⁰ have been analyzed by time-lapse spinning confocal microscopy in cells expressing Kif5C⁵⁶⁰-EGFP and control or Myo1b-mCherry-shRNA at DIV1 (A and B) or mCherry or mCherry-Myo1b (C and D). The merged first frames and the merged kymograph (one frame/2 min) for Kif5C⁵⁶⁰-EGFP (green) and mCherry (red) of the Video 4 (a and b) for shRNAs and Video 5 (a and b) for mCherry and mCherry-Myo1b are shown. T, time. Bars, 20 µm. The purple lanes on the first frame mark the regions for which the kymographs illustrate the dynamics of Kif5C⁵⁶⁰ within 1 h.