Figure 1.

Nanoorganization of β1 integrins within FAs. (A) Representative images of an Hs578T cell labeled by anti–total β1 integrin mAb K20 (OG488) acquired by confocal microscopy (left) and STED (middle). The white box in the middle panel is zoomed in the right inset. (B) Representative intensity profiles along an individual adhesion (green/red lines in A; n = 10, one profile per image) reveal distinct intensity peaks by STED (red) but not by confocal microscopy (green). (C) Spatial distribution of identified intensity peaks within adhesions in the STED image from A. (D) Representative image of an Hs578T cell labeled with anti–β1 integrin mAb K20 (Alexa Fluor 405 through Alexa Fluor 647) acquired by TIRF (left) and STORM (middle). The white box is zoomed in on the right and shows clusters identified by DBSCAN. Bars: (main images) 5 µm; (insets) 500 nm.