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. 2018 Jun 4;217(6):2167–2184. doi: 10.1083/jcb.201708053

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© 2018 Isensee et al.

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Figure 4. — Cell-permeable Rp-isomers inhibit the induction of pRII intensity and PKA downstream signaling in sensory neurons. (A–C) HCS microscopy analyses of Fsk-induced (10 µM, 5 min) pRII intensity after pretreatment of sensory neurons for up to 60 min with 10 µM Rp-cAMPS-pAB (A), Rp-8-pCPT-cAMPS-pAB (B), or Rp-8-Br-cAMPS-pAB (C). 10 µM 4-ABnOH served as negative control. (D–F) Dose–response experiments after 30 min pretreatment with Rp-isomers followed by stimulation with 10 µM Fsk (5 min). (G–I) Pretreatment with 10 µM Rp-cAMPS-pAB (30 min) effectively inhibited the pRII increase (G) and phosphorylation of CREB (H) and ERK1/2 (I). Values are means ± SEM; n = 3–4; >1,000 neurons/condition; two-way ANOVA with Bonferroni’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001 indicate significance levels between Fsk-stimulated conditions; ^§, P < 0.05; ^§§, P < 0.01; ^§§§, P < 0.001 indicate significance levels between basal conditions.