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. 2018 Jun 4;217(6):2073–2085. doi: 10.1083/jcb.201708115

Figure 3.

Figure 3.

SFT-4 is required for transport from the ER of CPL-1, but not SYN-1 or PGP-1. (A) ER export of CPL-1–YFP is impaired by RNAi of sft-4. Subcellular localization of CPL-1–YFP and DsRed-KDEL was examined in the intestines of mock- and sft-4(RNAi)–treated animals. (B) Intestines of transgenic animals expressing VIT-2–GFP were stained with an antibody against SYN-1. When sft-4 was depleted, SYN-1 was not markedly affected: although VIT-2–GFP was strongly accumulated in the ER (arrowheads), SYN-1 mainly localized to the basolateral membrane (arrows). (C) Subcellular localization of GFP–PGP-1 in the intestinal cells of mock- or sft-4(RNAi)–treated animals. GFP–PGP-1 was mostly transported to the apical plasma membrane even in sft-4(RNAi) animals. Dotted lines indicate the outlines of intestines. Regions surrounded by squares are enlarged (4× in A and 16× in B) in insets. Bars: 10 µm; (insets) 5 µm.