Figure 5.
Muscle-specific knockdown of β-PS-integrin promotes increased DNA content in myonuclei. Representative images of control (A and A′, Mef2-GAL4/yw) or β-PS-integrin knockdown (B and B′) muscle 7 labeled with phalloidin (red) and Hoechst (green). A′ and B′ are distinct focal planes at the level of muscle–muscle junction displaying similar magnification. Arrowheads demonstrate the adhesion defect induced by knockdown of β-PS-integrin (compare arrowheads in B′ to A′). Bars, 10 µm. (C) Quantification of the Hoechst integrated density of myonuclei per myofibers in muscle 7 indicates a significant increase in DNA content in β-PS-integrin knockdown versus control (t test: ****, P = 2.5 × 10−6). Images were taken from five distinct larvae (control, n = 14; integrin RNAi, n = 14). Whiskers extend to data points less than 1.5 interquartile ranges from the first and third quartiles.