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. 2018 Jun 4;217(6):2185–2204. doi: 10.1083/jcb.201708173

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© 2018 Hoefert et al.

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Figure 5. — Multiple miR-200 targets are involved with FA and AJ formation. (A) Immunofluorescence of vinculin and phalloidin stain for actin on WT keratinocytes infected with shScr, shCfl2, shEgfr, shRock2, shFak, or a combination of all shRNAs. (B) Quantification of FAs per cell in A. n = 34 Scr, n = 19 shCfl2, n = 26 shEgfr, n = 35 shFak, n = 20 shRock2, and n = 24 combined. (C) Immunofluorescence of E-cadherin and phalloidin stain for actin on WT keratinocytes infected with shScr, shCfl2, shEgfr, shRock2, shFak, or a combination of all shRNAs and induced with calcium for 3 or 7 h. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Student’s two-tailed t test. Bars, 20 µm.