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. 2018 Jun 4;217(6):2059–2071. doi: 10.1083/jcb.201709001

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© 2018 Lee et al.

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Figure 6. — Inhibitory effect of Fe²⁺ ions on Romo1 activity. (A) Inhibitory effect of H₂O₂ on Romo1-induced liposome permeabilization. CF-LUVs were incubated with the indicated concentrations of H₂O₂, and Romo1 (50 nM) was added for 10 min. (B–D) Determination of the inhibitory effects of transition metal ions on Romo1 activity. CF-LUVs were incubated with the indicated ions (10 µM), and Romo1 (50 nM) was added for 10 min. Alamethicin (Ala.; 0.5 µM) was used as a negative control (B). The IC₅₀ of Fe²⁺ was quantified by liposome permeabilization assay (C). CF-LUVs were incubated with the indicated ions (1 µM), and Romo1 (50 nM) was added for 10 min (D). Data represent means ± SD of three independent experiments. ***, P ≤ 0.001 by two-way ANOVA. Cont., control; D.W., distilled water.