Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 4;217(6):2047–2058. doi: 10.1083/jcb.201711151

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Chang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — EB1 binding constitutes a trapping mechanism limiting STIM1 localization at ER–PM junctions. (A) Fluorescence recovery of YFP-STIM1 and YFP-STIM1-TRNN after photobleaching (red square boxes) in HeLa cells, monitored by confocal microscopy. (B) Relative intensity of YFP-STIM1 and YFP-STIM1-TRNN in the bleached areas as described in A. 19–20 cells from three independent experiments. Mean times to the half recovery (t_1/2) are indicated. *, P < 0.05. (C and D) Changes in intensity of YFP-STIM1 (C) and YFP-STIM1-TRNN (D) at ER–PM junctions after 10 µM nocodazole (noc) treatment, monitored by TIRF microscopy in HeLa cells cotransfected with mCherry-ER. (E) Relative changes in intensity of STIM1 subtypes and mCherry-ER at ER–PM junctions derived from relative single puncta intensity as described in C and D. Intensity at the end time point is defined as 1. 13–14 cells from three to four independent experiments. (F) Localization of endogenous STIM1 and EB1 at resting state, visualized by immunostaining using confocal microscopy. (G) Fraction of STIM1 overlapping with EB1 calculated from quantification of endogenous (endo.) STIM1 and EB1 colocalization by immunostaining as described in F. Means ± SEM are shown. 29 cells from two independent experiments. (H) Changes in localization of YFP-STIM1 after 10 µM nocodazole treatment, monitored by confocal microscopy in HeLa cells stably expressing YFP-STIM1 at a low level. Bars: (A, F, and H) 10 µm; (C and D) 2 µm.