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. 2018 May 29;9:1135. doi: 10.3389/fmicb.2018.01135

Table 3.

Mutations inactivating oprD in carbapenem-non-susceptible isolates.

Type of mutation Mutational characteristicsa STs (no. of isolates)
Frameshift mutation 1-bp deletion (G) at nt 276 ST360 (19)
1-bp deletion (T) at nt 912 ST111 (22)
1-bp deletion (A) at nt 886 ST385 (1)
1-bp deletion (G) at nt 376 ST782 (1)
1-bp deletion (T) at nt 667 ST385 (3)
2-bp deletion (AT) at nt 1114-1115 ST316 (11), ST260 (4), ST2483 (4), ST485 (3), ST980 (2), ST274 (2), ST170 (1), ST270 (1), ST365 (1), ST499 (1), ST633 (1), ST782 (1), ST1028 (1), ST2420 (1), ST2492 (1), ST2493 (1), ST2494 (1)
2-bp deletion (TG) at nt 2-3 ST2479 (1)
10-bp deletion at nt 126-143 (CGACCTGCTGCTCCGCAA) ST385 (1)
10-bp deletion at nt 858-874 (TGCGCACACTTTCACCT) ST292 (1)
5-bp insertion (ATGGC) at nt 1054-1055 ST244 (4)
5-bp insertion (GGCCG) at nt 925-926 ST244 (3), ST595 (1)
7-bp insertion (CCTGTTC) at nt 469-470 ST244 (1)
Premature stop codon CAATAG at nt 199-201 ST316 (4)
GAA→TAA at nt 220-222 ST316 (3)
TGG→TAG at nt 16-18 ST639 (1)
TGG→TGA at nt 1015-1017 ST244 (2), ST385 (1)
CAG→TAG at nt 496-498 ST244 (1)
IS insertion IS1411 beginning at nt 788 ST1158 (13)
IS1394 beginning at nt -11 ST499 (4)
IS1394 beginning at nt -1 ST499 (1)
Amino acid substitution T103S, K115T, F170L ST277 (3), ST2488 (2), ST2478 (1)
T103S, K115T, F170L, E185Q, P186G, V189T, R310E, A315G, G425A ST245 (1), ST1648 (1)
No mutation None ST244 (2), ST554 (3), ST111 (1)
Negative by PCR Unknown change ST1158 (2)

IS, insertion sequence; nt, nucleotide. aSequences of oprD were compared to oprD in the reference strain PAO1. Bases in boldface indicate mutated nucleotides.