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. 2017 Jun 20;31(10):4305–4324. doi: 10.1096/fj.201700097R

Figure 5.

Figure 5.

A) The expression of miR-10a, -10b, -200b, and -335 increased in Mdr2−/− compared with WT mice and decreased in Mdr2−/− mice exposed to darkness. The expression of miR-29b decreased in Mdr2−/− compared with WT mice and increased in Mdr2−/− mice exposed to darkness. Data are means ± sem of 3 experments from cholangiocytes from 3 cumulative preparations from 4 mice (n = 12 mice). B) By miRNA quantitative PCR, miR-200b expression increased in cholangiocytes and hepatic stellate cells from Mdr2−/− mice but decreased in Mdr2−/− mice exposed to prolonged dark and treated with melatonin. Data are means ± sem of 6 experiments from 3 cumulative preparations of cholangiocytes from 4 mice (n = 12 mice); *P < 0.05 vs. WT mice; #P < 0.05 vs. Mdr2−/− mice. Expression of miR-200b was enhanced in human late-stage PSC samples compared with healthy control samples. The RNA was extracted from paraffin-embedded sections from samples obtained from 4 healthy control subjects and 4 male patients with late-stage PSC. Data are means ± sem of 3 different PCR reactions. *P < 0.05 vs. control samples. Melatonin decreased the expression in vitro of miR-200b in IMCLs compared with the basal value. Data are means ± sem of 3 evaluations of 3 individual preparations of IMCLs. *P < 0.05 vs. basal.