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. Author manuscript; available in PMC: 2018 Jun 5.
Published in final edited form as: ACS Nano. 2017 Dec 15;12(1):187–197. doi: 10.1021/acsnano.7b05528

Figure 2.

Figure 2

NP-siRNA are more cytotoxic in serum-free culture media. hMSCs were incubated for 24 hours with NPs complexed to a non-targeting negative control siRNA at varying concentrations at charge ratio (+/−) = 4 in DMEM + 10% FBS or serum-free OptiMEM media. At 30 nM siRNA, NP-siRNA complexes are more cytotoxic in OptiMEM compared to DMEM + 10% FBS. Cytotoxicity is observed in OptiMEM at concentrations > 10 nM siRNA. n=6; *p<0.05, ***p<0.001 compared to no treatment (NT); #p<0.0001 compared to 30 nM siRNA in DMEM + 10% FBS. Significance determined using two-way ANOVA with Dunnett’s test relative to NT control and Bonferroni’s test for 30 nM pairwise comparison. Error bars represent the standard deviation.