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. Author manuscript; available in PMC: 2018 Jun 5.
Published in final edited form as: ACS Nano. 2017 Dec 15;12(1):187–197. doi: 10.1021/acsnano.7b05528

Figure 4.

Figure 4

siRNA displacement from NPs is significantly reduced in the presence of serum. siRNA was complexed to NPs at a charge ratio (+/−) = 4 and incubated overnight with or without 10% FBS. Heparin, a poly(anion), was added to NP-siRNA complexes at varying concentrations to displace siRNA. Displaced (Free) siRNA was detected via gel electrophoresis (A), and quantified using ImageJ (B). n=3 gels for each condition; ****p<0.0001; Significance determined using two-way ANOVA with Bonferroni’s test to correct for multiple comparisons. Error bars represent the standard deviation.