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. Author manuscript; available in PMC: 2018 Jun 5.
Published in final edited form as: ACS Nano. 2017 Dec 15;12(1):187–197. doi: 10.1021/acsnano.7b05528

Figure 5.

Figure 5

NP-siRNA delivered in serum free media results in significantly more efficient gene silencing compared to NP-siRNA delivered in serum containing media. siRNA targeting human peptidylprolyl isomerase B (PPIB) gene was complexed to NPs at a charge ratio (+/−) = 4 and delivered to hMSCs in vitro in DMEM + 10% FBS or serum free OptiMEM media at varying concentrations and PPIB expression was detected via RT-PCR. PPIB expression is relative to no treatment (NT) groups and normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Dashed line represents gene silencing previously achieved using 30 nM NP-siRNA in DMEM + 10% FBS.22 n=6; ***p<0.001, ****p<0.0001 compared to no treatment (NT) control within a treatment group; #p<0.001; Significance determined using two-way ANOVA with Dunnett’s test to compare to no treatment (NT) control and Tukey’s test for multiple pairwise comparisons. Error bars represent the standard deviation.