Skip to main content
. Author manuscript; available in PMC: 2018 Jun 5.
Published in final edited form as: Cell Rep. 2018 May 1;23(5):1504–1515. doi: 10.1016/j.celrep.2018.03.135

Figure 3. Phosphorylation on Ste18 and Ste5 Cooperate to Prevent Early and Maximal Fus3 Activation.

Figure 3

Cells harboring the indicated combination of wild-type or mutant versions of Ste18 and Ste5 were stimulated with 3 μM α-F followed by quantitative immunoblot analysis of HA-Ste18 or activated Fus3 and Kss1.

(A) HA-Ste18 immunoblot in cells harboring wild-type (WT/WT) or Ste5ND (WT/non-docking [ND]).

(B) Quantitative comparison of pHA-Ste18 from (A) (n = 4).

(C) Representative immunoblot for activated Kss1 and Fus3.

(D) Quantitative comparison of activated Kss1 relative to wild-type peak activation at 5 min from immunoblots shown in (C).

(E) Quantitative comparison of activated Fus3 relative to wild-type peak activation at 30 min from immunoblots shown in (C). Data represent mean ± SD; n = 12. SE, short exposure; LE, long exposure; LC, loading control.

See also Figures S3, S4, and S5 and Table S2.