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. Author manuscript; available in PMC: 2018 Jun 5.
Published in final edited form as: Cell Rep. 2018 May 1;23(5):1504–1515. doi: 10.1016/j.celrep.2018.03.135

Figure 5. The Switch-like Morphological Response to Pheromone Is Regulated by Ste18-Nt Phosphorylation When Fus3 Cannot Bind to Ste5.

Figure 5

The morphological dose-response to mating pheromone represented by the cellular formation of a mating projection (i.e., shmoo) was quantified as a percentage of total cells in a population by DIC microscopy. Data were fit to a sigmodal dose-response curve with variable slope.

(A) Effect of individual Ste18Nt phosphorylation mutations on the mating response.

(B) Effect of Ste18Nt phosphorylation mutations in strains exclusively expressing the Fus3 non-docking mutant Ste5ND.

Error bars represent SEM across ≥200 cells per experiment.

See also Table S3.