Figure 4.

Clonogenic assay shows the long-term effects of CH-AuNPs treatment of HeLa and MCF-7 cells.

Notes: HeLa (A) and MCF-7 (B) cells were treated with CC₂₅ and CC₅₀ of CH-AuNPs for 24 hours, the number of colonies formed on the culture plate 10 days after treatment was expressed as surviving fractions. Data are reported as percentage of colonies compared with untreated control (left). Photograph of petri-dish of a representative experiment is shown (right).

Abbreviations: CH-AuNPs, chitosan gold nanoparticles; CC₂₅, concentration required to reduce cell viability by 25%; CC₅₀, concentration required to reduce cell viability by 50%.