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. 2018 May 31;13:3235–3250. doi: 10.2147/IJN.S165289

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© 2018 Martínez-Torres et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Caspase-3 activity and effects of pan-caspase inhibition on CH-AuNPs-treated HeLa and MCF-7 cells.

Notes: Caspase-3 activation was measured by flow cytometry in HeLa (A) and MCF-7 (B) cells after treatment with CH-AuNPs for 24 hours (left). Data were then analyzed and graphed (right). Cell viability was determined by flow cytometry in HeLa (C) and MCF-7 (D) cells that were left alone or pretreated with QVD and then treated with CC₅₀ of CH-AuNPs for 24 hours (left). The percentages of the graph bars (right) refer to AnnV-positive and/or PI-positive staining.

Abbreviations: AnnV, Annexin-V; CH-AuNPs, chitosan gold nanoparticles; PI, propidium iodide; QVD, quinoline-val-asp-difluorophenoxymethyl ketone.