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. 2018 Jun 4;150(6):851–862. doi: 10.1085/jgp.201711968

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© 2018 Cherny et al.

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Figure 4. — Gating of His¹⁶⁸ mutants has normal pH_o dependence. (A–C) Families of currents in a cell expressing H168Q with pH_i 6 and pH_o 5, 6, and 7, with pulses in 10-mV increments up to the voltage indicated. Holding potential, V_hold was −40 mV (A and B) or −60 mV (C). (D and E) Proton current–voltage curves (D) and g_H-V relationships (E) from the families in A–C exhibit a normal 40-mV shift/unit change in pH_o.