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. 2018 Jun 4;150(6):809–819. doi: 10.1085/jgp.201711974

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© 2018 Reda and Chandra

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 5. — Effect of TnT_F88L on E_R at short and long SLs. E_R corresponds to the ML-mediated increase in steady-state force and represents the magnitude of ML-mediated XB recruitment. E_R is estimated as the slope of the linear relationship between (F_nss−F_ss) and ML changes, ΔL (see Figs. 1 and 4, A and B). Estimates from several muscle fibers per group were averaged and presented as mean ± SEM. Two-way ANOVA revealed a significant TnT–SL interaction effect (P = 0.031) on E_R. Post hoc multiple pairwise comparisons (Fisher’s LSD) were used to determine significant differences between groups. Asterisks indicate significant difference from TnT_WT at a given SL (*, P < 0.05). The numbers of fibers measured (from three hearts) for TnT_WT and TnT_F88L at short SL were 9 and 10, and those at long SL were 10 and 10, respectively.