Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 4;215(6):1679–1692. doi: 10.1084/jem.20172048

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Cao et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — ZA is a direct inhibitor of UGT8 and inhibits breast cancer cell migration and invasion. (A and B) Expression of GalCer and sulfatide was measured by immunofluorescent staining in MDA-MB231 and SUM159 cells treated with or without ZA (20 µM; A), HCC1937 cells with empty vector, or stable UGT8 expression as well as UGT8-expressing HCC1937 cells treated with or without ZA (20 µM; B). Nuclei were visualized with DAPI (blue). Bars, 20 µm. (C) In vitro activity assay of UGT8 was performed by mixing UDP-galactose, substrate, and lysate of MDA-MB231 cells. After treatment of the indicated concentration of ZA, UDP-galactose consumption and UDP production were tested by HPLC system. The percentage of UDP-galactose consumption and UDP production was shown in the bar graph (mean ± SD in three separate experiments). (D) Expression of GalCer and sulfatide was examined by immunoblotting in MDA-MB231 and SUM159 cells treated with the indicated concentration of ZA. (E and F) Migratory ability (E) and invasiveness (F) of MDA-MB231 and SUM159 cells treated with the indicated concentration of ZA. The percentage of migratory and invasive cells was shown in the bar graph (mean ± SD in three separate experiments). *, P < 0.05 by Student’s t test.