Figure 7.

Knockdown of UGT8 expression inhibits tumorigenicity in vitro and in vivo. (A) Soft-agar assay was performed using MDA-MB231, SUM159, MDA-MB435, and MDA-MB436 cells with stable empty vector or knockdown of UGT8 expression (A), as well as BT549, HCC1937, and BT20 cells with stable empty vector or UGT8 expression (B). Data are presented as a percentage of empty vector cell lines (mean ± SD in three separate experiments). (C and D) MDA-MB231 cells (C) and SUM159 cells (D) with stable empty vector or knockdown of UGT8 expression were injected into the mammary fat pad of SCID mice. The growth of tumors was examined every 2 d. Tumor size and weight were recorded. Data are shown as mean ± SEM from six mice. *, P < 0.001. (E) Box plots indicated UGT8 expression in different tumor size of breast cancer from NKI295 dataset. Comparisons were made using the two-tailed Student’s t test. (F) Box plots indicated UGT8 expression in different histological grades of breast cancer from multiple datasets (NKI295, GSE25066, and GSE1456). Comparisons between two groups were made using the two-tailed Student’s t test.