Figure 8.

Inhibition of UGT8 suppresses metastasis in vivo and elevated UGT8 predicts poor survival. (A and B) MDA-MB231 cells (A) and MDA-MB231 cells with stable empty vector or knockdown of UGT8 expression (B) were injected into SCID mice via the tail vein. For evaluation of ZA, the mice received ZA (0.0186 mg/kg/d) or sterile PBS subcutaneously. After 4 wk, the development of lung metastases was monitored using bioluminescence imaging and quantified by measuring photon flux (mean of six animals + SEM; left). Three representative mice from each group were shown (middle). Lung metastatic nodules were examined in paraffin-embedded sections stained with hematoxylin and eosin. The arrowheads indicate lung metastases. Bar, 100 µm (A, right). (C–E) Kaplan-Meier survival analysis for overall survival (OS), RFS, and distant metastasis-free survival (DMFS) of patients in the NKI295 dataset according to UGT8 expression status. The p-value was determined using the log-rank test. (F and G) Kaplan-Meier survival analysis for RFS of patients with various subtypes (F) or BLBC (G) in an aggregate breast cancer dataset according to UGT8 expression status. The p-value was determined using the log-rank test.