Figure 7.

Correlations of antibody binding responses in early and late chronic infection. (A and B) Pairwise Spearman correlation analysis of the relative binding activities to the 13 HIV-1 antigens within and between subclass IgG1 (dark green), IgG2 (light green), and IgG3 (gray) in white subtype B infected patients with early chronic (1–3 yr untreated infection; n = 519; A) and late chronic (>3 yr untreated infection; n = 2,243; B) infection. Correlations with a magnitude >0.15 are depicted in blue (positive associations) and red (negative associations). The strength of Spearman correlation is signified by color intensity for correlations with magnitudes from 0.15 to 0.5 and by the strongest color intensity and line width for magnitudes >0.5. (C) Significant differences (p_shuffle < 0.05; 10,000 random reshuffles) in pairwise Spearman correlations between early chronic (as in A) and late chronic (as in B) patients are shown. Green and orange shaded lines denote correlations that increase or decrease among late chronic patients, respectively. The significance level of differences is depicted by color intensity and by line width for differences that remain significant after correction for multiple testing (Benjamini-Hochberg correction with a false discovery rate of 10%; p_shuffle ≤ 0.0148). (D) The distribution of all pairwise “between IgG subclass” correlations of individual antigens (Fig. 5 E) is shown for the early chronic (green) and the late chronic (violet) patients. Significant p-values from a paired Wilcoxon test are shown.