What does this study involve doing to the animals?	What will the animals experience? How much suffering might it cause? What might make it worse Adverse effects	How will suffering be reduced to a minimum
		Methodology and interventions to minimise severity	End-points
Pre-surgical preparation of the animal	Single housing prior to surgery Food withdrawal overnight prior to surgery		NA
Surgical implantation of telemetry devices	Possible pain and discomfort Potential adverse effects of anaesthesia and surgery (hypothermia, dehydration, distress on recovery	Appropriate perioperative care: appropriate anaesthesia and analgesia, etc. Non-pharmacological control of pain and distress: aseptic technique, heating, well-trained personnel, optimised surgical procedure	Decision to humanely kill the animal during surgery if unacceptable complications arise and persist
Period of recovery (3 weeks), including training	Possible pain, infection, discomfort Post-operative complications Single housing during post-operative period (24 h) and training (2 h a day)	Adequate post-operative care (analgesics, antibiotics) as needed Daily observation and wound care	Any animal with clinical signs of infection will be examined, treated and temporarily excluded from the study
Administration of test compound (i.v., s.c., i.p., p.o.) before recording parameters	Possibly transient discomfort following administration of compound Potential compound-related unexpected side effects Animals are individually housed for short-term periods (determined by the pharmacokinetics of the drug).	Blood pressure, ECG and body temperature are continuously monitored in freely moving animal, housed in pen equipped with telemetry receiver. Between studies animals are group housed and observed daily, Body weight is recorded weekly.	In case of prolonged side effects after test compound treatment: possibly treat and allow animal longer wash-out period and clinical check-up before new dosing and decision on possible euthanasia. End of battery life: animal is either proposed for re-use as blood donor, or if competent authorities authorise, explanted and depending on vet examination proposed for rehoming or proposed for re-use as blood donor.