Fig. 2.

Kinetics of uptake of tenofovir for species orthologs of OAT1. (A) The uptake kinetics of [³H]-tenofovir in HEK293 cells expressing hOAT1, cyOAT1, rat OAT1, mouse OAT1, and dog OAT1. The uptake rate was evaluated at 3 minutes. Each point represents the mean ± S.D. uptake in the OAT1-transfected cells minus that in empty vector cells. (B) Correlation of the V_max value of OAT1-mediated tenofovir uptake in HEK293 cells stably expressing five OAT1 species orthologs with total cell membrane-bound OAT1 protein quantity (R² = 0.892).