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. 2018 Jul;366(1):46–57. doi: 10.1124/jpet.117.247262

Fig. 6.

Fig. 6.

NOX4−/− BM-MSCs from 9- to 10-week-old male and female mice develop fewer fibroblast colonies but are protected against EtOH’s suppression of AP. (A) Count of hematoxylin-positive fibroblast colonies (CFU-F) per million cells plated. (B) Percentage of hematoxylin-positive colonies that stained positive for AP. (C) Representative images of WT (top) and NOX4−/− (bottom) cultures treated with or without EtOH, following staining for AP using Fast Blue (WT) or Fast Violet (NOX4−/−) salt. Arrow: representative punctate AP + CFU-F. (D) Percentage of hematoxylin-positive colonies that stained positive for AP (p47: cultures extracted from p47phox−/− animals lacking functional NOX2). Statistical significance was determined by two-way ANOVA; values with different letter superscripts are statistically different from each other (P < 0.05, Tukey’s multiple comparisons analysis). For each group: WT 0 mM EtOH, n = 15; WT 50 mM EtOH, n = 12; NOX4−/− 0 mM, n = 7; NOX4−/− 50 mM, n = 7.