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. Author manuscript; available in PMC: 2018 Jun 5.
Published in final edited form as: Cell Rep. 2018 May 15;23(7):1939–1947. doi: 10.1016/j.celrep.2018.04.036

Figure 3. Loss of Donor Cell DHSs Is Associated with Downregulation of Somatic Cell Transcription Program.

Figure 3

(A and B) Heatmap showing the dynamics of OC (A) and OO (B) DHSs during preimplantation development analyzed by liDNase-seq (left) and ATAC-seq (right). Each row represents a locus (DHS center ± 5 kb), and the red and blue gradient colors represent the liDNase-seq and ATAC-seq signal intensity, respectively. OC DHSs were separated into two groups based on whether they reappear at the morula stage (FC[morula/cumulus] > 3, RPKM > 1, and mean RPKM > 2 in morula embryos). Peaks were ordered based on the signal intensity in cumulus cells.

(C) Averaged gene expression levels of the OO- or OC-associated genes (TSS ± 10 kb) in cumulus cells and preimplantation embryos. The p values were calculated using the Wilcoxon rank sum test.

(D) Averaged gene expression levels of the CO-associated genes (TSS ± 10 kb) in cumulus cells and preimplantation embryos. The p values were calculated using the paired t test.

See also Figure S3.