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. Author manuscript; available in PMC: 2018 Jun 5.
Published in final edited form as: Cell Rep. 2018 May 15;23(7):1939–1947. doi: 10.1016/j.celrep.2018.04.036

Figure 4. DHS Reprogramming in SCNT Is Independent of DNA Replication.

Figure 4

(A) Schematic illustration of the experiment to examine the effect of aphidicolin on SCNT-mediated DHS reprogramming.

(B) Representative images of SCNT one-cell embryos immunostained with BrdU and lamin B1 antibodies. No BrdU signal was detected in aphidicolin-treated embryos. The number of SCNT embryos exhibiting the staining pattern and the total number of embryos analyzed are shown, respectively. Scale bar: 20 μm.

(C) PCA of the genome-wide DHS profile of cumulus, IVF(Pat), SCNT, and aphidicolin-treated SCNT samples. Each dot represents an independent sample.

(D) Heatmap showing liDNase-seq enrichment signal in the OC and CO DHSs after aphidicolin treatment. Each row represents a locus (DHS center ± 5 kb), and the red gradient color indicates the signal intensity.

See also Figure S4.