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. 2018 Jun 5;13(6):e0197362. doi: 10.1371/journal.pone.0197362

Fig 7. Depletion of intracellular calcium or inhibition of PKC blocks NNK induced trypsinogen activation.

Fig 7

(A) Trypsinogen activation by NNK (100 nM) was not inhibited in calcium free media. But, preincubation with the membrane permeable calcium chelator BAPTA-AM (10 μM, 30 min) followed by switching to a calcium free media significantly inhibited trypsinogen activation. (B) NNK induced trypsinogen activation was inhibited by preincubation with the broad-spectrum PCK inhibitor GF-109203X (10 μM) for 120 min. Values are means ± SE; n = 3. *P < 0.05 vs. control, #P < 0.05 vs. NNK alone.