Table 1. Selected genes used for real-time qPCR studies.
Selected genes related to the oxidative stress control, homologies, primer sequences, access number on NCBI GenBank and amplicon size (bp).
| Gene Symbol | Primer Sequence (5'-3') | Gene Description | NCBI GenBank Access Number | Amplicon Size (bp) |
|---|---|---|---|---|
| UBQ2* | F: GATGATACTTGGCCCTGCAC | Ubiquitin-conjugating enzyme E2 | GR984245 | 142 |
| R: CCTTCCCAGCTTGTCAATGT | ||||
| APXc | F: GATTGCCTTTTGCTGTCTGATG | Putative cytosolic ascorbate peroxidase (cAPX) | JQ013438.1 | 132 |
| R: CGGGAATATGAACGACCACATA | ||||
| APXm | F: GAACTGGGTTTTACTCCACATTCC | Membrane-bound ascorbate peroxidase (mAPX) mRNA | JQ013439.1 | 119 |
| R: CAAGTAACTGAGAACCACAACTGC | ||||
| APXt+s | F: AGGGCAGAATATGAAGGATTGG | Stromatic ascorbate peroxidase (sAPX) mRNA | JQ013441.1 | 112 |
| R: CCAAGCAAGGATGTCAAAATAGCC | ||||
| PX4 | F: CCAAGTTCTTATGAGCGACAACAC | Putative class III peroxidase (POX4) | JQ013435.1 | 106 |
| R: TGCCCATCTTTACCATTGACAC | ||||
| VDE2 | F: GGGTTCAAAATGCACAAGACTG | Violaxanthin de-epoxidase | DQ234768.1 | 86 |
| R: CCCTCTTTTACCTCAGGCATTG |
* Used to check for DNA contamination in RNA samples and positive control for cDNA synthesis.