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. 2018 Jun 5;13(6):e0198694. doi: 10.1371/journal.pone.0198694

Table 3. Values of leaf relative water content (RWC, %) and water potential (ΨW, MPa).

Values were obtained at predawn along the entire experiment for Apoatã, Icatu, and Obatã genotypes, under well-watered (WW), mild drought (MD) and severe drought (SD) conditions, and submitted to temperature control conditions (25/20 oC), during the gradual temperature decrease (18/13 oC), at the end of the acclimation period (13/8 oC), after 3 chilling cycles (3x13/4 oC), after 7 days under rewarming conditions (7x Rec Cold), and after a further 7 days period under rewatering conditions (7x Rec Drought).

Genotype Treatment Temperature (day/night)
25/20°C 18/13°C 13/8°C 3x13/4°C 7x Rec Cold 7x Rec Drought
Predawn RWC (%)
WW 92.1 ± 3.3 aA 94.5 ± 1.4 aA 79.2 ± 6.8 bcAB 73.0 ± 5.4 cA 90.9 ± 0.5 abA 91.8 ± 0.7 aA
Apoatã MD 88.4 ± 2.8 abAB 85.0 ± 1.5 abAB 83.8 ± 2.8 abA 77.2 ± 4.9 bA 82.5 ± 4.1 abA 89.4 ± 2.7 aA
SD 82.9 ± 3.1 abB 78.2 ± 6.5 abcB 72.0 ± 5.4 bcB 69.1 ± 6.5 cA 82.2 ± 3.1 abA 84.5 ± 2.3 aA
WW 92.3 ± 1.8 abA 88.9 ± 2.2 abA 87.3 ± 2.1 abA 81.1 ± 3.2 bA 88.3 ± 3.4 abA 92.4 ± 0.9 aA
Icatu MD 79.7 ± 2.6 abB 80.7 ± 1.8 abB 77.3 ± 2.0 bB 73.8 ± 2.0 bAB 78.2 ± 3.0 bB 89.1 ± 1.0 aA
SD 69.5 ± 3.4 bC 63.0 ± 2.7 cC 71.0 ± 1.1 bB 72.3 ± 0.8 bB 64.4 ± 3.8 bcC 89.0 ± 0.9 aA
WW 91.2 ± 1.1 aA 89.0 ± 1.6 aA 85.2 ± 2.2 aA 83.5 ± 2.3 aA 86.3 ± 2.6 aA 87.3 ± 1.5 aA
Obatã MD 86.5 ± 1.1 aAB 83.8 ± 2.4 aAB 81.2 ± 1.4 aAB 82.6 ± 2.1 aA 79.4 ± 1.5 aAB 86.7 ± 0.7 aA
SD 82.8 ± 2.7 abB 74.6 ± 4.9 bB 74.0 ± 2.9 bB 79.2 ± 3.0 aA 77.3 ± 1.6 abB 85.6 ± 1.6 aA
Predawn ΨW (MPa)
WW -0.42 ± 0.06 aA -0.54 ± 0.06 aA -0.66 ± 0.10 aA -0.63 ± 0.09 aA -0.41 ± 0.05 aA -0.42 ± 0.06 aA
Apoatã MD -0.72 ± 0.07 abAB -0.88 ± 0.05 abA -1.22 ± 0.14 bAB -0.84 ± 0.09 abA -0.83 ± 0.28 abAB -0.43 ± 0.03 aA
SD -1.02 ± 0.24 abB -1.51 ± 0.21 bB -1.61 ± 0.31 bB -1.06 ± 0.03 abA -1.32 ± 0.45 bB -0.47 ± 0.04 aA
WW -0.40 ± 0.04 aA -0.56 ± 0.07 aA -0.85 ± 0.12 aA -0.49 ± 0.12 aA -0.37 ± 0.04 aA -0.37 ± 0.03 aA
Icatu MD -1.11 ± 0.04 bB -1.46 ± 0.22 bcB -1.99 ± 0.10 cdB -2.22 ± 0.18 dB -2.16 ± 0.09 dB -0.40 ± 0.05 aA
SD -2.86 ± 0.07 bC -3.13 ± 0.39 bC -2.96 ± 0.28 bC -2.95 ± 0.27 bC -3.24 ± 0.36 bC -0.42 ± 0.03 aA
WW -0.46 ± 0.05 aA -0.56 ± 0.06 aA -0.81 ± 0.09 aA -0.55 ± 0.04 aA -0.37 ± 0.04 aA -0.45 ± 0.06 aA
Obatã MD -0.86 ± 0.12 abAB -0.98 ± 0.21 abA -1.83 ± 0.14 cB -1.31 ± 0.15 bcB -1.35 ± 0.24 bcB -0.45 ± 0.03 aA
SD -1.33 ± 0.21 bB -2.10 ± 0.09 cB -2.49 ± 0.12 cC -2.01 ± 0.09 cC -2.12 ± 0.32 cC -0.56 ± 0.06 aA

For each parameter, the mean values ± SE (n = 5) followed by different letters express significant differences between temperature treatments for the same water availability level (a, b, c), or between water treatments for each temperature treatment (A, B, C), always separately for each genotype.