Table 7. Changes in gene transcription.
Real-time-qPCR expression values (n fold) relative to the expression value observed under control conditions of temperature (25/20°C) and water availability (WW), within each genotype. The values are for the entire experiment from leaves of Apoatã, Icatu, and Obatã genotypes, under well-watered (WW), mild drought (MD) and severe drought (SD) conditions, and submitted to temperature control conditions (25/20 oC), at the end of the acclimation period (13/8 oC), after 3 chilling cycles (3x13/4 oC), after 7 days under rewarming conditions (7x Rec Cold), and after a further 7 days period under rewatering conditions (7x Rec Drought). It were studied genes of the enzymes ascorbate peroxidases from cytosolic ascorbate peroxidase (APXc), membrane-bound ascorbate peroxidase (APXm), and stromatic ascorbate peroxidase (APXt+s), peroxidase (PX4), and violaxanthin de-epoxidase (VDE2).
| Gene Expression Relative to Control Conditions | |||||||
|---|---|---|---|---|---|---|---|
| Genotype | Temperature | Water | APXc | APXm | APXt+s | PX4 | VDE2 |
| Apoatã | 25/20°C | WW | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| MD | 2.96 | 2.76 | 3.03 | 3.13 | 2.84 | ||
| SD | 2.85 | 2.20 | 2.13 | 5.73* | 4.07 | ||
| 13/8°C | WW | 1.37 | 1.40 | 1.54 | 2.87 | 0.86 | |
| MD | 3.16 | 2.62 | 4.45* | 6.34* | 1.69 | ||
| SD | 0.67 | 0.57 | 0.69 | 3.75 | 3.63 | ||
| 3 x 13/4°C | WW | 1.53 | 1.05 | 1.54 | 0.12* | 0.92 | |
| MD | 2.02 | 2.55 | 2.24 | 1.31 | 0.30 | ||
| SD | 1.42 | 1.02 | 1.89 | 1.24 | 1.46 | ||
| 7x Rec Cold | WW | 3.90 | 1.78 | 3.80 | 6.95* | 1.52 | |
| MD | 7.02* | 3.01 | 3.93 | 2.43 | 2.61 | ||
| SD | 2.29 | 1.76 | 2.28 | 4.39 | 1.80 | ||
| 7x Rec Drought | WW | 2.15 | 1.59 | 1.80 | 6.98* | 1.13 | |
| MD | 3.53 | 2.61 | 1.82 | 8.16* | 1.97 | ||
| SD | 3.54 | 1.79 | 1.82 | 9.42* | 2.64 | ||
| Icatu | 25/20°C | WW | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| MD | 6.43* | 1.39 | 4.18* | 15.24* | 0.25 | ||
| SD | 6.63* | 0.76 | 1.75 | 0.67 | 0.24 | ||
| 13/8°C | WW | 1.86 | 0.32 | 1.16 | 2.11 | 0.15 | |
| MD | 2.20 | 0.31 | 1.53 | 6.56* | 0.07* | ||
| SD | 10.28* | 1.91 | 3.22 | 10.54* | 0.69 | ||
| 3 x 13/4°C | WW | 1.05 | 0.21 | 0.74 | 2.30 | 0.07* | |
| MD | 2.88 | 0.41 | 1.12 | 3.55 | 0.09* | ||
| SD | 3.98* | 0.72 | 2.27 | 8.50* | 0.14 | ||
| 7x Rec Cold | WW | 1.20 | 0.30 | 0.86 | 4.60 | 0.15 | |
| MD | 1.84 | 0.31 | 0.83 | 2.67 | 0.11* | ||
| SD | 5.51* | 0.69 | 2.60 | 4.86* | 0.45 | ||
| 7x Rec Drought | WW | 0.92 | 0.15* | 0.47 | 4.27 | 0.23 | |
| MD | 3.17 | 0.20 | 0.77 | 7.68* | 0.29 | ||
| SD | 7.54* | 0.31 | 1.66 | 9.93* | 0.10* | ||
| Obatã | 25/20°C | WW | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| MD | 1.45 | 1.34 | 2.29 | 0.71 | 1.61 | ||
| SD | 0.92 | 1.03 | 0.29 | 0.59 | 1.19 | ||
| 13/8°C | WW | 0.88 | 0.91 | 1.42 | 1.70 | 1.05 | |
| MD | 1.56 | 0.95 | 2.15 | 0.50 | 0.54 | ||
| SD | 1.03 | 1.60 | 0.29 | 0.65 | 0.52 | ||
| 3 x 13/4°C | WW | 0.64 | 0.72 | 1.20 | 0.60 | 0.46 | |
| MD | 1.21 | 1.00 | 1.97 | 1.64 | 0.74 | ||
| SD | 2.15 | 1.12 | 0.40 | 0.59 | 0.57 | ||
| 7x Rec Cold | WW | 1.00 | 0.68 | 1.23 | 0.44 | 0.46 | |
| MD | 0.96 | 0.51 | 1.08 | 0.22 | 0.61 | ||
| SD | 0.93 | 0.71 | 0.26 | 0.27 | 0.93 | ||
| 7x Rec Drought | WW | 0.96 | 0.51 | 1.11 | 1.02 | 0.36 | |
| MD | 1.65 | 0.93 | 1.22 | 1.16 | 0.75 | ||
| SD | 1.79 | 0.98 | 0.53 | 1.42 | 1.07 | ||
Original expression values for each gene resulted from the mean ± SE (n = 6–9), from 3 independent biological assays.
* indicate the presence of statistical significance.