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. Author manuscript; available in PMC: 2018 Jun 5.
Published in final edited form as: Sci Signal. 2017 Nov 7;10(504):eaan0852. doi: 10.1126/scisignal.aan0852

Fig. 4. Constitutively active cofilin rescues aberrant spine morphology and density In the somatosensory cortex of young Fmr1 KO mice.

Fig. 4

(A) Experimental timeline of viral application on cultured neurons or viral delivery and stereotaxic injection into the somatosensory cortex. (B) Representative Western blots and summary data of the F-actin/G-actin ratio in lysates from somatosensory cortical cultures (10 to 12 DIV) either unperturbed or infected with virus expressing GFP, WT cofilin, constitutively active coflinS3A, or phosphomimetic cofilinS3D (n = 13 to 15 culture wells per group from three independent experiments). (C) Representative fluorescent images assessing viral-mediated transduction of apical dendrites of layer V somatosensory cortex pyramidal neurons with GFP (left), GFP-WT cofilin (middle), and GFP-cofilinS3A (right) in male WT and Fmr1 KO mice at P10 to P12. Scale bar, 2 μm. Examples of mature spines (arrowheads) and immature protrusions (arrows) are indicated. (D to I) Summary data of average spine length (D), head width (E), spine length-to-width ratio (LWR) (F), % mature (stubby/mushroom) spines (G), % immature (thin/filopodia) spines (H), and spine density (I) of the samples imaged in (C) (n = 15 to 21 neurons pooled from 6 to 10 animals per group). Data are means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.