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Raman spectra of Rnase at different purification steps (range 1200–1800 cm−1). a. Native Rnase (blue spectrum); b. Rnase-TCA (red spectrum) (shifted 1500 arbitrary units (a. u.) downward); c. Rnase-ethanol/HCl (black spectrum) (shifted 600 a. u. upward).
