Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| genetic reagent (Drosophila melanogaster) |
Or47b-GAL4 | (Fishilevich and Vosshall, 2005) | RRID:BDSC_9984 | |
| genetic reagent (D. melanogaster) |
Or22a-GAL4 | (Dobritsa et al., 2003) | RRID:BDSC_9951 | |
| genetic reagent (D. melanogaster) |
10XUAS-myc-APEX2-Orco | this study | see Materials and methods | |
| genetic reagent (D. melanogaster) |
10XUAS-mCD8GFP-APEX2 | this study | see Materials and methods | |
| genetic reagent (Mus musculus) |
B6.Cg-Crhtm1(cre)Zjh/J | Jackson Laboratory | 031559 RRID:IMSR_JAX:031559 |
|
| genetic reagent (M. musculus) |
B6.Cg-Gt.ROSA.26 Sortm14(CAG-tdTomato)Hze/J |
Jackson Laboratory | 007914 RRID:IMSR_JAX:007914 |
|
| genetic reagent (M. musculus) |
TH-GFP | (Kessler et al., 2003) | ||
| cell line | HEK 293T | ATCC | CRL-3216 RRID:CVCL_0063 |
|
| antibody | DRAQ5 | Cell Signaling Technology | 4084 | 1:1000 |
| recombinant DNA reagent (plasmids) |
pcDNA3-Mito-V5-APEX2 | (Lam et al., 2015) | Addgene_72480 | |
| recombinant DNA reagent (plasmids) |
APEX2 DNA | (Lam et al., 2015) | Addgene_49386 | |
| recombinant DNA reagent (plasmids) |
APEX2-Orco | this study | see Materials and methods | |
| recombinant DNA reagent (plasmids) |
mCD8GFP-APEX2 | this study | see Materials and methods | |
| chemical compound, drug | paraformaldehyde | Fisher Scientific | 04042–500 | |
| chemical compound, drug | glutaraldehyde | Ted Pella | 18426 | |
| chemical compound, drug | sodium cacodylate | Ted Pella | 18851 | |
| chemical compound, drug | CaCl2 | Sigma-Aldrich | 223506 | |
| chemical compound, drug | glycine | Bio-Rad Laboratories | 161–0718 | |
| chemical compound, drug | BSA | Sigma-Aldrich | 9048-46-8 | |
| chemical compound, drug | 1-hexadecene | Sigma-Aldrich | H2131 | |
| chemical compound, drug | uranyl acetate | Electron Microscopy Sciences | 22400 | |
| chemical compound, drug | methanol | Fisher Scientific | A412-4 | |
| chemical compound, drug | acetone | ACROS Organics | AC326800010 | |
| chemical compound, drug | HEPES | Gibco | 15-630-080 | |
| chemical compound, drug | diaminobenzidine; DAB | Sigma-Aldrich | D5637 | |
| chemical compound, drug | H2O2 | Fisher Scientific | H325-100 | |
| chemical compound, drug | osmium tetroxide; OsO4 | Electron Microscopy Sciences | 19190 | |
| chemical compound, drug | potassium ferrocyanide | Mallinckrodt | 6932 | |
| chemical compound, drug | thiocarbohydrazide | Electron Microscopy Sciences | 21900 | |
| chemical compound, drug | Durcupan ACM resin component A | Sigma-Aldrich | 44611 | |
| chemical compound, drug | Durcupan ACM resin component B | Sigma-Aldrich | 44612 | |
| chemical compound, drug | Durcupan ACM resin component C | Sigma-Aldrich | 44613 | |
| chemical compound, drug | Durcupan ACM resin component D | Sigma-Aldrich | 44614 | |
| chemical compound, drug | conductive silver epoxy | Ted Pella | 16043 | |
| software, algorithm | IMOD | (Kremer et al., 1996) | RRID:SCR_003297 | http://bio3d.colorado.edu/imod/ |
| software, algorithm | ImageJ | NIH | RRID:SCR_003070 | https://imagej.nih.gov/ij/ |
| software, algorithm | Amira 6.3 | ThermoFisher | RRID:SCR_014305 | |
| other | Aclar | Electron Microscopy Sciences | 50426 | |
| other | FocusClear | Cedarlane Labs | FC-101 |