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. 2018 Jun 5;7:e33800. doi: 10.7554/eLife.33800

Figure 3. PAX3 and PAX3-FOXO1 induce distinct gene expression signatures during development, identifying a unique PAX3-FOXO1 target, her3.

Figure 3.

Microarray analysis identified differentially expressed gene signatures in 24 hr old zebrafish FACS sorted embryonic cells that expressed either GFP2A-PAX3FOXO1 or GFP2A-PAX3. (A) Gene ontology terms associated with PAX3 or PAX3-FOXO1. (B) Embryonic tissues indicative of PAX3 or PAX3-FOXO1 gene sets. (C) 3D principal components analysis (PCA) of GFP injected controls, PAX3, and PAX3-FOXO1. (D) Intersection of up-regulated genes for PAX3 and PAX3-FOXO1 as compared to GFP controls. Uniquely up-regulated PAX3-FOXO1 target genes were included in downstream analysis only if they possessed a human ortholog. Shown are genes rank ordered based on the fold-change of their expression. (E) qRT-PCR of her3 levels from 24-hr-old zebrafish embryos that are either uninjected controls or injected with GFP, PAX3, or PAX3-FOXO1. (F) qRT-PCR for PAX3-FOXO1 mRNA levels from 24-hr-old zebrafish embryos injected with PAX3-FOXO1, or PAX3-FOXO1 in combination with a GFP morpholino (GFP MO) that inhibits transgene expression. (G) Same samples as in F, but qRT-PCR was performed for her3. In E-G the SD is derived from technical triplicates, * indicates p<0.05, two-tailed Student’s t-test.