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(A) A case with negative HER-2 amplification status, HER-2/CEP17 ratio <2, DAKO Paraffin Pretreatment Kit (4’,6-diamidino-2-phenylindole [DAPI] counterstain,×1000); (B) A case with positive HER-2 amplification status, HER-2/CEP17 > 2, DAKO Paraffin Pretreatment Kit (DAPI counterstain, ×1000); (C) Stromal cells (left half) were used as the internal control to tumor cells (right half), DAKO Paraffin Pretreatment Kit (DAPI counterstain, ×1000).
