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. 2018 Jun 4;28(11):1783–1793.e4. doi: 10.1016/j.cub.2018.04.040
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Anti-GFP (rabbit) Life Technologies Cat#A11122
Anti-HA (rat) Roche/Sigma Cat#11867423001
Anti-nc82 (mouse) DSHB Cat# nc82, RRID:AB_2314866
Anti-rat AlexA 594 Life Technologies Cat#A11007
Anti-rabbit AlexA 488 Life Technologies Cat#A11034
Anti-mouse AlexA 647 Life Technologies Cat#A21235
Chemicals, Peptides, and Recombinant Proteins
4-methylcyclohexanol (98%) Sigma-Aldrich Cat#218405
3-octanol (99%) Sigma-Aldrich Cat#153095
Paraffine GPR Rectapur VWR Cat#24679.360
Bovin Serum Albumin EuroMedex Cat#04-100-812-C
PFA Electron Microscopy Science Cat#15710
Triton X-100 Sigma-Aldrich Cat#93443
Prolong Mounting Medium Life Technologies Cat#P36965
NaCl Sigma-Aldrich Cat#S9625
KCl Sigma-Aldrich Cat#P3911
MgCl2 Sigma-Aldrich Cat#M9272
CaCl2 Sigma-Aldrich Cat#3881
ribose Sigma-Aldrich Cat#W379301
HEPES-NaOH Sigma-Aldrich Cat#H7637
Sucrose Sigma-Aldrich Cat#S9378
DNaseI New England Biolabs Cat#M0303S
Critical Commercial Assays
RNeasy Plant Mini Kit QIAGEN Cat#74904
RNA Minielute Cleanup kit QIAGEN Cat#74204
SuperScript III First-Strand kit Thermofisher Invitrogen Cat#18080-051
SYBR Green I Master mix Roche Cat#04729692001
Experimental Models: Organisms/Strains
MB112C-split GAL4 [3] N/A
R83A12-Gal4 BDSC N/A
30E11-LexA BDSC N/A
30E11-LexA;R83A12-Gal4 This paper N/A
NP2758-GAL4 [20] N/A
NP0047-Gal4 [12] N/A
Tubulin-GAL80ts line as described in [20]] BDSC N/A
Tubulin-GAL80ts;NP0047-Gal4 This paper N/A
Tubulin-GAL80ts;NP2758-Gal4 This paper N/A
Tubulin-GAL80ts;R83A12-Gal4 This paper N/A
UAS-Shits [12] N/A
UAS-TrpA1 [12] N/A
UAS-dDA1RNA1 (KK102341) VDRC VDRC v107058
UAS-GABABR1RNAi1 (KK109166) VDRC VDRC v101440
UAS-DAMBRNAi2 (KK110947) VDRC VDRC v105324
UAS-DAMBRNAi1 (JF02043) BDSC BDSC 26018
UAS-DopEcRRNAi1( KK111211) VDRC VDRC v103494
UAS-dD2RRNAi1 (JF02025) BDSC BDSC 26001
UAS-GABABR1RNAi2 (HMC03388) BDSC BDSC 51817
AOP-GCaMP3 Barret Pfeiffer (Janelia Research Center) N/A
UAS-IVS-GCaMP3 in VK00005 Barret Pfeiffer (Janelia Research Center) N/A
AOP-GCaMP3, UAS-Shi This paper N/A
UAS-GABABR1RNAi1(KK109166);UAS-IVS-GCaMP3 This paper N/A
UAS-Syt-HA;; Pr. Hiromu Tanimoto (Tohoku University, Japan) N/A
;;AOP-mCD8::GFP BDSC BDSC 32203
UAS-Syt-HA;;AOP-mCD8::GFP This paper N/A
Oligonucleotides
Primer GABA BR1 forward: 5′TTGGATGATGTCAACAAGCAG −3′ This Paper N/A
Primer GABA B R1 reverse: 5′TTTTGCGGTTTATTATAGAGCAGAT −3′ This Paper N/A
Primer Tubulin forward: 5′-TTGTCGCGTGTGAAACACTTC-3′ [56] N/A
Primer Tubulin forward: 5′-CTGGACACCAGCCTGACCAAC-3′ [56] N/A
Software and Algorithms
GraphPad Prism 5 GraphPad Software Inc. Press, San Diego CA, 2007 http://www.graphpad.com/
MATLAB V2013 The Mathworks Inc., Natick, MA https://fr.mathworks.com/
Fiji NIH https://imagej.net/Fiji