Fig. 2.

Binding analysis of 1H6-G4 nucleic acids interaction after A) 1H6 direct amine coupling, B) anti-mouse mediated 1H6 capturing, C) anti-mouse mediated capturing of the 1H6-G4 complex. Oxy2 G4 analyte was tested in the concentration range 62.50 nM–8.00 μM, while for un3 and bcl-2 the 250 nM–8.00 μM range was sufficient to cover the kinetic spectrum. Recorded sensorgrams are shown in red, while fitting curves are in black. Fitting curves were not reported when they did not fit or the kinetic constants were outside the sensitivity of the instrument. Parameters for the goodness of fittings are reported in Table S1.