Table 1.
Kinetic parameters for 1H6-G4 interaction.
| Amine coupling |
G4 Ab capturing |
G4 Ab/G4 capturing |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ka (Ms−1) | kd (s−1) | KD (nM) | ka (104 Ms.−1) | kd (10−4 s−1) | KD (nM) | ka (104 Ms.−1) | kd (10−4 s−1) | KD (nM) | ||
| Oxy2 | nd | nd | nd | 29.50 ± 0.92 | 6.99 ± 1.73 | 26.13 ± 0.51 | 5.83 ± 0.40 | 5.13 ± 1.59 | 10.78 ± 3.38 | tetra |
| 110.60 ± 56.40 | 2385.26 ± 1774.46 | 74.45 ± 3.85 | 96.30 ± 41.91 | 438.73 ± 141.40 | 64.33 ± 15.15 | bi | ||||
| un3 | nd | nd | nd | nd | nd | nd | 0.07 ± 0.01 | 3.30 ± 0.10 | 479.65 ± 2.75 | |
| bcl-2 | nd | nd | nd | nd | nd | nd | 0.58 ± 0.01 | 30.10 ± 11,0 | 518.05 ± 20.20 | |
nd = non detectable; uncertainties of the reported values are calculated as the standard deviation of at least two experimental replicates obtained with independent ligand immobilizations.